- 528 Hz is a frequency, and this means that something or a certain note repeats 528 times per second. In music tuning to frequency means tuning one note on an instrument to that specific frequency. So if you tune your violin and for example to 440 Hz, then you are tuning the A string so that when played, IT vibrates at a frequency of 440 oscillations per second. The Myth Of 528 Hz Pitch Explained (naturesoundretreat.com)